纖溶酶原激活物抑制因子2(PAI2)活性蛋白
Active Plasminogen Activator Inhibitor 2 (PAI2)
SERPINB2; PLANH2; Serpin Peptidase Inhibitor Clade B Member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor
活性實驗
Serpin B2, also known as PAI-2, is an approximately 60 kDa serine protease inhibitor. It is primarily secreted by macrophages and monocytes and can form disulfide-linked multimers. Serpin B2 inhibits both the urokinase-type and tissue-type plasminogen activators (uPA and tPA). Serpin B2 also promotes the clearance of uPA by enhancing its binding and uptake by LRP. It limits fibril formation by Huntington protein (HTT) and beta-Amyloid peptides. It promotes Th2 biased immune responses and is important for intestinal CCL2 production, monocyte recruitment, and nematode clearance. A non-glycosylated form of Serpin B2 is retained intracellularly where it interferes with TNF-a induced apoptosis by protecting the Retinoblastoma protein (RB1) from calpain digestion. It also inhibits proteasome activity in activated endothelial cells. The activity of recombinant mouse PAI-2 was measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC) in the assay buffer 50 mM Tris, 0.01% Tween 20, pH 8.5. The 50 ul different concentrations of rmPAI-2 (MW: 47.9 KD) was incubated with 50ul 2ug/ml rhuPA (EPA140Mu61) at room temperature for 15?minutes. Loading 50?μL of the incubated mixtures into empty wells of a plate, and start the reaction by adding 50?μL of 200?μM substrate (Z-GGR-AMC). Include a substrate blank containing 50?μL of assay buffer and 50?μL of 200?μM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that PAI2 significantly decreased uPA activity. The inhibition IC50 was <300 nM.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。
增值服務
相關產品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
APA531Mu61 | 纖溶酶原激活物抑制因子2(PAI2)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA531Mu02 | 纖溶酶原激活物抑制因子2(PAI2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA531Mu61 | 纖溶酶原激活物抑制因子2(PAI2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA531Mu01 | 纖溶酶原激活物抑制因子2(PAI2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA531Mu01 | 纖溶酶原激活物抑制因子2(PAI2)多克隆抗體 | WB; IHC; ICC; IP. |
PAA531Mu02 | 纖溶酶原激活物抑制因子2(PAI2)多克隆抗體 | WB; IHC; ICC; IP. |
SEA531Mu | 纖溶酶原激活物抑制因子2(PAI2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA531Mu | 纖溶酶原激活物抑制因子2(PAI2)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
BBA Clinical | Cerebrospinal fluid inflammatory markers in patients with?Listeria monocytogenes?meningitis [Sciencedirect:S2214647414000087] |
JCI Insight | The senescence-associated secretome as an indicator of age and medical risk [Pubmed: 32554926] |